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KMID : 0880220080460030350
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Journal of Microbiology
2008 Volume.46 No. 3 p.350 ~ p.355
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Molecular Analysis of the fur (ferric uptake regulator) Gene of a Pathogenic Edwardsiella tarda Strain
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Wang Fang
Cheng Shuang
Sun Kun
Sun Li
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Abstract
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The gene encoding the Edwardsiella tarda ferric uptake regulator (FurEt) was cloned from a pathogenic E. tarda strain isolated from diseased fish. FurEt shares 90% overall sequence identity with the Escherichia coli Fur (FurEc) and was able to complement the mutant phenotype of a furEc-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated FurEt whereas E112K mutation resulted in a superactive FurEt variant. FurEt negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence.
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KEYWORD
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Edwardsiella tarda, Fur, autoregulation, virulence, mutation
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